Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, Porcine, Ch | IH(P), IP, WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS2028-100UG |
| Replaces | 04-1526 |
| Description | Anti-Vitamin D receptor Antibody, clone 9A7 |
| Alternate Names |
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| Background Information | Vitamin D3 receptor (UniProt: O42392; also known as VDR, 1,25-dihydroxyvitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1) is encoded by the VDR (also known as NR1I1) gene (Gene ID: 395988) in chicken. VDR is a nuclear receptor for calcitriol, the active form of vitamin D3 that mediates the action of vitamin D on cells. VDR is expressed mainly in kidney and intestine and plays a central role in calcium homeostasis. In the absence of bound vitamin D3 it is present as a homodimer and it enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. It is recruited to promoters via its interaction with BAZ1B/WSTF, which mediates the interaction with acetylated histones that is considered to be an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. VDR is composed of three domains: a modulating N-terminal domain, a DNA-binding domain, and a C-terminal ligand-binding domain. Two isoforms of VDR have been described that are produced by alternative initiation. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Vitamin D receptor, clone 9A7, Cat. No. MABS2028, is a rat monoclonal antibody that detects Vitamin D3 receptor and has been tested for use in , Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot detected Vitamin D receptor in Immunoprecipitation applications (Pike, J.W., et. al. (1982). Proc Natl Acad Sci USA. 79(24); 7719-23). Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Vitamin D receptor in human skin and human breast cancer tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | Partially purified chicken intestinal vitamin D receptor. |
| Clone | 9A7 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 9A7 detects Vitamin D3 receptor in multiple avian and mammalian species. |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Species Reactivity Note | Human, Chicken, Porcine, Rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~51 kDa observed; 51.3 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in MCF-7 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Vitamin D receptor in MCF-7 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |