Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS2039-25UG |
| Description | Anti-SUMO-2/3 Antibody, clone 8A2 |
| Alternate Names |
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| Background Information | Small ubiquitin-related modifier 2 (UniProt P61956; also known as HSMT3, Sentrin-2, SMT3 homolog 2, Smt3B, SUMO-2, Ubiquitin-like protein SMT3B) and 3 (UniProt P55854; also known as SMT3 homolog 1, Smt3A, SUMO-3, Ubiquitin-like protein SMT3A) are encoded by the SUMO2 (also known as SMT3B, SMT3H2; Gene ID 6613) and SUMO3 (also known as SMT3A, SMT3H1; Gene ID 6612) genes in human. SUMOylation, protein post-translation modification by small ubiquitin-like modifier (SUMO), is a signaling event in many cellular processes. SUMO proteins are translated as immature precursors and subsequently converted to their mature forms through the activity of sentrin/SUMO-specific proteases (SENPs). SUMOylation is a reversible process. SUMO E1 activating enzyme, E2 conjugating enzyme, and E3 ligase mediate SUMOylation of substrate proteins, while SENPs are responsible for the de-SUMOylation. SUMOylation usually occurs at lysine residues in the consensus KxD/E motif, although not all such lysines become SUMOylated and SUMOylation can also occur on lysine residues outside of this motif. SUMO2 and 3 share 97% identity at the amino acid level, while SUMO1 shares only about 50% sequence homology with SUMO-2 and 3. In addition to difference in their target substrates, SUMO2/3 can be SUMOylated and form chains, whereas SUMO1 cannot and may serve as chain terminator. SUMO-2 can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Its covalent attachment to its substrate via an isopeptide bond requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Although SUMO-2 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system, however, unlike ubiquitin which targets proteins for degradation, SUMO-2 is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus (aa 94-95; propeptide) have been cleaved off. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-SUMO-2/3, clone 8A2, Cat. No. MABS2039, is a mouse monoclonal antibody that detects SUMO-2 and SUMO-3 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected SUMO-2/3 in Western Blotting applications (Zhang, X.D., et. al. (2008). Mol Cell. 29(6):729-41; Zhu, S., et. al. (2009). Mol Cell. 33(5):570-80). Immunoprecipitation Analysis: A representative lot detected SUMO-2/3 in Immunoprecipitation applications (Zhu, S., et. al. (2009). Mol Cell. 33(5):570-80). Western Blotting Analysis: A 1:500 dilution from a representative lot detected SUMO-2/3 in HeLa cell lysate (Courtesy of Christine Lee, Matunis Lab, John Hopkins University, Baltimore, Maryland USA). Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected SUMO-2/3 in HeLa cells (Courtesy of Christine Lee, Matunis Lab, John Hopkins University, Baltimore, Maryland USA). Immunocytochemistry Analysis: A representative lot detected SUMO-2/3 in Immunocytochemistry applications (Zhang, X.D., et. al. (2008). Mol Cell. 29(6):729-41; Rao, H.B., et. al. (2017) Science. 355(6323):403-407). Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected SUMO-2/3 in HeLa cells. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged full length recombinant human SUMO-2 protein. |
| Clone | 8A2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 8A2 detects SUMO-2/3 in human cells. |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~19 kDa observed; 10.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 4 µg/mL of this antibody detected SUMO-2/3 in HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |