Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P), FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS2045-100UG |
| Description | Anti-HPa2 Antibody, clone DHIC2-2B4 |
| Alternate Names |
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| Background Information | Pancreas performs both the endocrine and the exocrine function. The bulk of pancreas is composed of exocrine cells that produce digestion enzymes which are released into ducts to be emptied into the duodenum. The endocrine pancreas is composed of islets of Langerhans that produce hormones. At least five distinct endocrine cell types have been identified in the endocrine pancreas, each producing a different hormone. Beta cells produce insulin, which is considered as an anabolic hormone and regulates blood glucose levels and is also involved in fat and protein synthesis. Alpha cells produce glucagon that acts when blood glucose levels are low. Ductal cells of pancreas form the epithelial lining of the branched tubes that deliver digestive enzymes into the duodenum. They also secrete bicarbonate to neutralize acidic contents coming in from stomach. Duct cells may contribute up to 10% of all pancreatic cells. Acinar cells function as a unit of the exocrine pancreas and they synthesize, store, and secrete digestive enzymes (lipase, alpha amylase, trypsin, and chymotrypsin), which under normal physiological conditions are activated only after reaching the duodenum. Premature activation of these enzymes within pancreatic acinar cells can lead to acute pancreatitis. Clone DHIC2-2B4 can be used to detect alpha cells in human pancreatic islets. (Ref.: Dorrell, C., et al. (2008). Stem Cell Research (2008). 1(3); 183-194; Grapin-Botton, A (2005). Int. J. Biochem. Cell Biol. 37(3); 504-510; Leung, PS (2006). Int. J. Biochem. Cell Biol. 38(7); 1024-1030). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Detects Alpha cells of human pancreatic islets using this mouse monoclonal Anti-HPa2, clone DHIC2-2B4, Cat. No. MABS2045, tested for use in Immunohistochemistry (Paraffin) and Flow Cytometry. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot detected HPa2 in Immunohistochemistry applications (Dorrell, C., et. al. (2008). Stem Cell Res. 1(3):183-94). Flow Cytometry Analysis: A representative lot detected HPa2 in Flow Cytometry applications (Dorrell, C., et. al. (2008). Stem Cell Res. 1(3):183-94; Bramswig, N.C., et. al. (2013). J Clin Invest. 123(3):1275-84; Dorrell, C., et. al. (2016). Nat Commun. 7:11756). |
| Biological Information | |
|---|---|
| Immunogen | Trypsin-dissociated human pancreatic islet cells containing low levels of exocrine and ductal cells. |
| Clone | DHIC2-2B4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone DHIC2-2B4 specifically detects alpha cells in human pancreatic tissue sections. |
| Isotype | IgG1λ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in alpha cells of human pancreatic tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected HPa2 in alpha cells of human pancreatic tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |