Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, Ht | ELISA, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS2046-25UG |
| Description | Anti-ApoB Antibody, clone 2G11 |
| Alternate Names |
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| Background Information | Apolipoprotein B-100 (UniProt: E9Q414; also known as Apo B-100) is encoded by the Apob gene (Gene ID: 238055) in murine species. Apolipoprotein B is a major protein constituent of chylomicrons. Apo B particles are made up of lipids and proteins and each Apo B is bordered by a phospholipid monolayer with an inner core composed of variable amounts of triglycerides and cholesterol esters. Apo B is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off. Two isoforms of Apo B are produced- Apo B-100 is synthesized in the liver and Apo B-48 is produced in the intestine. Apo B-100 contains the region that binds to the LDL receptor. It contains VLDL particles, VLDL remnant particles and LDL particles. On the other hand, the Apo B-48 particles are made of chylomicrons and chylomicron remnant particles. Generally, LDL particles account for approximately 90% of Apo B particles in plasma. Abnormal trapping of an Apo B is a major factor in the development of atherosclerotic lesions. When Apo B particles degrade cholesterol is retained with macrophages that induce inflammatory response. Further entry and entrapment of Apo B can promote cardiovascular pathology over time. (Ref.: Sniderman, A et Al. (2010). Nat. Rev. Endocrinol. 6(6); 335-346). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Detects Apolipoprotein B using this mouse monoclonal Anti-ApoB, clone 2G11 , Cat. No. MABS2046, is a mouse monoclonal antibody that detects Apolipoprotein B in Mouse, Rat, and Hamster and is tested for use in ELISA and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 4 µg/mL from a representative lot detected ApoB in 10 µg of rat liver and mouse liver tissue lysate. Western Blotting Analysis: A representative lot detected ApoB in Western Blotting applications (Nguyen, A.T., et. Al. (2006). Biochem Biophys Acta. 1761(2):182-5; Cheng, D., et. Al. (2016). J Biol Chem. 291(45):23793-23803). ELISA Analysis: A representative lot detected ApoB in ELISA applications (Nguyen, A.T., et. Al. (2006). Biochem Biophys Acta. 1761(2):182-5). |
| Biological Information | |
|---|---|
| Immunogen | ApoB-containing lipoproteins isolated from Apoe -/- Apob 48/48 mice. |
| Clone | 2G11 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 2G11 detects Apolipoprotein B in Mouse, Rat, and Hamster plasma and tissues. It binds to ApoB-48 and ApoB-100, but not to ApoB-39. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Mouse, Rat, Hamster. Does not react with human. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~260 kDa observed; 509.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat serum. Western Blotting Analysis: 4 µg/mL of this antibody detected ApoB in 10 µg of rat serum. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |