| Description | |
|---|---|
| Catalogue Number | MABS2049-100UG |
| Description | Anti-Adenylate Cyclase 5 Antibody, clone 19D5.C1 |
| Alternate Names |
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| Background Information | Adenylate cyclase type 5 (P84309; also known as EC: 4.6.1.1, ATP pyrophosphate-lyase 5, Adenylate cyclase type V, Adenylyl cyclase 5, AC5) is encoded by the Adcy5 gene (Gene ID: 224129) in murine species. Adenylate cyclase catalyzes the formation of cyclic AMP in response to g-protein signaling. It regulates the increase of free cytosolic calcium in response to increased blood glucose levels and contributes to the regulation of calcium-dependent insulin secretion. Adenylyl cyclases are typically made up of two clusters of six transmembrane domains separating two intracellular, overlapping catalytic domains. The amino acid sequence of each cytoplasmic domain that contains the nucleotide (ATP)-binding site, is well conserved among various subtypes. Thus far 9 different hormone-sensitive, membrane-bound AC isoforms have been identified. Adenylyl Cyclase 5 deficiency is shown to protect against diet-induced obesity and insulin resistance in mice and extends their life span. Adenylate cyclase 5 knockout mice display signs of reduced stress and protection against the stress of chronic pressure overload on heart. Adenylate cyclase 5 activity is stimulated by forskolin and is inhibited by adenosine and ATP analogs. (Ref.: Ho, D., et Al. (2015). Diabetes. 64(7); 2636-2645. Vatner, SF., et Al. (2015). Oxid. Med. Cell. Longev. 2015:250310). |
| Product Information | |
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| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
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| Application | Anti-Adenylate Cyclase 5, clone 19D5.C1, Cat. No. MABS2049, is a mouse monoclonal antibody that detects adenylate cyclase 5 and has been tested for use in ELISA and Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: A representative lot detected Adenylyl Cyclase 5 in Western Blotting applications (Hu, C.L., et. Al. (2009). Am J Physiol Heart Circ Physiol. 297(5):H1776-82; De Lorenzo, M.S., et. Al. (2014). Aging Cell. 13(1):102-10). ELISA Analysis: A representative lot detected Adenylyl Cyclase 5 in ELISA applications (Hu, C.L., et. Al. (2009). Am J Physiol Heart Circ Physiol. 297(5):H1776-82). |
| Biological Information | |
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| Immunogen | KLH-conjugated linear peptide corresponding to 20 amino acids from the cytoplasmic domain of murine adenylate cyclase V. |
| Epitope | cytoplasmic domain |
| Clone | 19D5.C1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 19D5.C1 detects Adnylate Cyclase 5 in murine, rat, and procine cells. It targets an epitope within 20 amino acids from the cytoplasmic domain. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Mouse, Rat, Porcine. Predicted to react with Human based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~150 kDa observed; 139.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting in mouse brain membrane preparations. Western Blotting Analysis: 1 µg/mL of this antibody detected Adenylate Cyclase 5 in mouse brain membrane preparations. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 100 μg |