Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ICC, IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS2066-25UG |
| Description | Anti-LLGL1 Antibody, clone mAb17-35 |
| Alternate Names |
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| Background Information | Lethal(2) giant larvae protein homolog 1 (UniProt: Q80Y17; also known as LLGL, Mgl-1, Mlgl) is encoded by the Llgl1 (also known as Llglh) gene (Gene ID: 16897) in murine species. LLGL belongs to the WD repeat L(2)GL family. LLGL is a cortical cytoskeleton protein that is found in a complex involved in maintenance of cell polarity and epithelial integrity. It is involved in the regulation of mitotic spindle orientation, proliferation, differentiation, and tissue organization of neuroepithelial cells. It is generally enriched in developing axons. LLGL stability and function is achieve by its interaction with RanBPM protein, which primarily acts as a scaffolding protein. Its N-terminal region containing SPRY domain is shown to be responsible for binding to RanBPM and this binding functionally extends its half-life by preventing its turnover through ubiquitin-proteasomal pathway. RanBPM is shown enhance tumor suppressor activity of LLGL by inhibiting LLGL-mediated cell proliferation and cell migration. (Ref.: Suresh, B., et Al. (2010). J. Biol. Chem. 285(46); 35340-35349). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-LLGL1, clone mAb17-35, Cat. No. MABS2066, is a mouse monoclonal antibody that detects Lethal(2) giant larvae protein homolog 1 and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected LLGL1 in Western Blotting applications (Ivanov, A.I., et. Al. (2010). Am J Pathol. 176(1):134-45). Immunocytochemistry Analysis: A representative lot detected LLGL1 in Immunocytochemistry applications (Ivanov, A.I., et. Al. (2010). Am J Pathol. 176(1):134-45). Immunohistochemistry Analysis: A representative lot detected LLGL1 in Immunohistochemistry applications (Weimer, J.M., et. Al. (2009). Development. 136(17):2965-75). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to 153 amino acids from the C-terminal region of murine Lethal(2) giant larvae protein homolog 1 (LLGL). |
| Epitope | C-terminus |
| Clone | mAb17-35 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone mAb17-35 specifically detects LLGL1 in mouse. It targets an epitope with in 153 amino acids from the C-terminal region. |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~120 kDa observed; 112.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse brain tissue lysate. Western Blotting Analysis: 4 µg/mL of this antibody detected LLGL1 in mouse brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |