Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Zebrafish, R, Ch, Xn | ICC, IHC, IF, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS2071-25UG |
| Description | Anti-SUMO-1 Antibody, clone 21C7 |
| Alternate Names |
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| Background Information | Small ubiquitin-related modifier 1 (UniProt: P63165; also known as SUMO-1, GAP-modifying protein 1, GMP1, SMT3 homolog 3, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Smt3C, Ubiquitin-like protein UBL1) is encoded by the SUMO1 (also known as SMT3C, SMT3H3, UBL1, OK/SW-cl.43) gene (Gene ID: 7341) in human. SUMO-1 is a ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in many cellular processes, such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SUMO-1 can also covalently attach to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1. Polymeric SUMO-1 chains are shown to be susceptible to polyubiquitination, which functions as a signal for proteasomal degradation of modified proteins. Two isoforms of SUMO-1 have been reported that are produced by alternative splicing. Mutations in SUMO1 gene are known to cause non-syndromic orofacial cleft 10, which is a birth defect consisting of cleft lips with or without cleft palate. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-SUMO-1, clone 21C7, Cat. No. MABS2071, is a mouse monoclonal antibody that detects Small ubiquitin-related modifier 1 (SUMO-1) and has been tested for use in Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot detected SUMO-1 in Immunohistochemistry applications (Rogers, R.S., et. Al. (2004). Chromosoma. 113(5):233-43; Costa, M.W., et. Al. (2011). PLoA One. 6(9):e24812). Immunocytochemistry Analysis: A representative lot detected SUMO-1 in Immunocytochemistry applications (Matunis, M.J., et. Al. (1996). J Cell Biol. 135(6 Pt 1):1457-70; Zhang, X.D., et. Al. (2008). Mol Cell. 29(6):729-41). Immunoprecipitation Analysis: A representative lot immunopprecipitated SUMO-1 in Immunoprecipitation applications (Costa, M.W., et. Al. (2011). PLoA One. 6(9):e24812; Becker, J., et. Al. (2013). Nat Struct Mol Biol. 20(4):525-31; Girach, F., et. Al. (2013). Cell Rep. 5(5):1294-301; Chanda, A., et. Al. (2017). PLoS One. 12(5):e0177639). Western Blotting Analysis: A representative lot detected SUMO-1 in Western Blotting applications (Zhang, X.D., et. Al. (2008). Mol Cell. 29(6):729-41; Matunis, M.J., et. Al. (1996). J Cell Biol. 135(6 Pt 1):1457-70; Rogers, R.S., et. Al. (2004). Chromosoma. 113(5):233-43; Costa, M.W., et. Al. (2011). PLoA One. 6(9):e24812; Becker, J., et. Al. (2013). Nat Struct Mol Biol. 20(4):525-31). Immunofluorescence Analysis: A representative lot detected SUMO-1 in Immunofluorescence applications (Girach, F., et. Al. (2013). Cell Rep. 5(5):1294-301; Rogers, R.S., et. Al. (2004). Chromosoma. 113(5):233-43; Matunis, M.J., et. Al. (1996). J Cell Biol. 135(6 Pt 1):1457-70). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged full length human recombinant Small ubiquitin-related modifier 1 (SUMO-1). |
| Clone | 21C7 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 21C7 detects SUMO-1 in multiple species. It targets an epitope with in 11 amino acids from the internal region. |
| Isotype | IgG2b |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse, Zebra Fish, Rat, Chicken, Xenopus. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~17 kDa observed; 11.56 kDa calculated. SUMO-1/RanGAP1 band is observed AT ~ 75 kDa. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HCT116 cell lysate. Western Blotting Analysis: 4 µg/mL of this antibody detected SUMO-1 in HCT116 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |