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Millipore Anti-Ppm1a, Clone 7f12 Antibody Is An Antibody Against Ppm1a For Use In Western Blotting, Flow Cytometry & ELISA. - Mill (Additional S&H or Hazmat Fees May Apply)
Protein phosphatase 1A, also known as Protein phosphatase 2C isoform alpha (PP2C-alpha) or Protein phosphatase IA, and encoded by the gene name PPM1A, is a member of the PP2C family. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal in PBS containing up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-PPM1A, clone 7F12 Antibody is an antibody against PPM1A for use in western blotting, flow cytometry & ELISA.
Key Applications
Western Blotting
Flow Cytometry
ELISA
Application Notes
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected PPM1A in HeLa cells.
ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Biological Information
Immunogen
Purified recombinant fragment of human PPM1A expressed in E. Coli
Clone
7F12
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Gene Symbol
PPM1A
PPPM1A
Purification Method
Protein g Purified
UniProt Number
P35813
Molecular Weight
~43 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Jurkat, A431, HeLa, Raji, MCF-7 and COS7 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PPM1A in Jurkat, A431, HeLa, Raji, MCF-7 and COS7 cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
