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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS443 |
| Description | Anti-GRP78 Antibody, clone C38 |
| Alternate Names |
|
| Background Information | The gene HSPA5 encodes a protein named GRP-78 (78 kDa glucose-regulated protein) also known as BiP (immunoglobulin heavy chain-binding protein) or heat shock 70 kDa protein 5. While originally discovered as one of set of genes that were induced upon cellular starvation and stress, HSPA5 encoded GRP-78 is now known to be an important member of the heat-shock family of proteins. The HSPA5 encoded GRP-78 protein appears to play a role in helping in the assembly of multimeric protein complexes in the endoplasmic reticulum (ER). Cellular localization is in the endoplasmic reticulum lumen and appears as cytoplasmic-like staining. The HSPA5 encoded protein is an essential component of the translocation processes within the endoplasmic reticulum and also functions in the retrograde transport across the ER membrane of incorrectly folded proteins destined to be degraded by the ubiquitin proteasome system. The HSPA5 encoded protein, while widely expressed in all cells types under normal conditions, is particularly induced under conditions of cellular stress, heat, or toxins and conditions which lead to the accumulation of unfolded proteins in the ER. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GRP78 Antibody, clone C38 detects level of GRP78 & has been published & validated for use in GRP78. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A representative lot from an independent laboratory detected GRP78 in HepG2 cells (de Ridder, g. g., et Al. (2012). Melanoma Res. 22(3):225-235.). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human GRP78. |
| Clone | C38 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | 72 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in HeLa cells. Flow Cytometry Analysis: 1 µg of this antibody detected GRP78 in 1X10E6 HeLa cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |