| Description | |
|---|---|
| Catalogue Number | MABT1322-100UG |
| Description | Anti-GCP2 Antibody, clone GCP2-02 |
| Alternate Names |
|
| Background Information | Gamma-tubulin complex component 2 (UniProt: Q921G8; also known as GCP2) is encoded by the Tubgcp2 (also known as Gcp2) gene (Gene ID: 74237) in murine species. Gamma-tubulin complex that is essential for microtubule nucleation at the centrosome is composed of gamma-tubulin, GCP2, GCP3, GCP4, GCP5 and GCP6. GCPs are characterized by sequence homology in two specific regions known as the grip1 and grip2 motifs. GPCs associate with gamma-tubulin to form a V-shaped subcomplex known as gamma-tubulin small complex. In this complex GCPs 2 and 3 interact via their N-terminal domain and form the arms of the V. The two-distal part of the V (C-terminal domains) bind to gamma-tubulin. The gamma-tubulin complex serves as a template for polarized growth and regulation of microtubules in eukaryotes. Significantly higher expression of both GPC2 and 3 have been reported in glioblastoma cells and they are concentrated I the centrosomes in interphase glioblastoma cells. Depletion of GPC2 and 3 causes accumulation of cells in G2/M and result in mitotic delay. (Ref.: Farache, D., et Al. (2016). J. Biol. Chem. 291(44); 23112-25; Draberova, E., et Al. (2015). J. Neuropathol. Exp. Neurol. 74(7); 723-42). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-GCP2, clone GCP2-02, Cat. No. MABT1322, is a mouse monoclonal antibody that detects Gamma-tubulin complex component 2 (CGP2) and has been tested for use in ELISA, Electron Microscopy, Immunocytochemistry, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1 µg/mL from a representative lot detected GCP2 in human glioblastoma T98G cell lysate. Immunoprecipitation Analysis: A representative lot immunoprecipitated GCP2 in Immunoprecipitation applications (Draberova, E., et. Al. (2015). J Neuropathol Exp Neurol. 74(7):723-42). Immunocytochemistry Analysis: A representative lot detected GCP2 in Immunocytochemistry applications (Draberova, E., et. Al. (2015). J Neuropathol Exp Neurol. 74(7):723-42). Western Blotting Analysis: A representative lot detected GCP2 in Western Blotting applications (Draberova, E., et. Al. (2015). J Neuropathol Exp Neurol. 74(7):723-42). ELISA Analysis: A representative lot detected GCP2 in ELISA applications (Draberova, E., et. Al. (2015). J Neuropathol Exp Neurol. 74(7):723-42). Electron Microscopy Analysis: A representative lot detected GCP2 in Electron Microscopy applications (Draberova, E., et. Al. (2015). J Neuropathol Exp Neurol. 74(7):723-42). Immunoprecipitation Analysis: A represnetative lot immunoprecipitated GCP2 in mouse bone-marrow mast cells (Courtesy of Pavel Draber, pH.D., Institute of Molecular Genetics of the ASCR, v.v.i., Prague, Czech Republic). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to the first 194 amino acids from the N-terminal region of murine Gamma-tubulin complex component 2. |
| Clone | GCP2-02 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone GCP2-02 specifically detects GCP2 in T98G glioblastoma cells. It targets an epitope within 12 amino acids from the N-terminal region. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Chicken, Fish, Frog. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein g purified |
| UniProt Number |
|
| Molecular Weight | ~100 kDa observed; 103.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in T98G human glioblastoma cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected GCP2 in lysate from T98G human glioblastoma cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |