Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT1323-25UL |
| Description | Anti-Cdh1 Antibody, clone AR38.2 |
| Alternate Names |
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| Background Information | Fizzy-related protein homolog (UniProt: Q9R1K5; also known as Fzr, Cdh1/Hct1 homolog) is encoded by the Fzr1 (also known as Fyr, Fzr) gene (Gene ID: 56371) in murine species. Cdh1 is a substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex that associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. APC/C is activated upon sequential binding to the Cdc20 and Cdh1 adaptor proteins. Cdc20 associates with APC/C during early mitosis and principally regulates mitotic progression, while Cdh1 interacts with the APC/C from late mitosis onwards until the following G1/S transition. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition Cdh1 is phosphorylated, leading to its dissociation from the APC/C. It has been shown that Cdk-mediated Cdh1 phosphorylation prevents Cdh1 from associating with APC/C prematurely during S/G2 phase, while CDC14B phosphatase-dependent APC/C-Cdh1 activation in response to DNA damage during G2 phase leads to ubiquitination and degradation of Polo-like kinase 1 (Plk1) and prevents entry into mitosis. Following DNA repair, Cdk1 and Plk1 are reactivated and cell cycle progresses from G2 into mitosis phase. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Cdh1, clone AR38, Cat. No. MABT1323, is a mouse monoclonal antibody that detects Cdh1/FZR1 and has been tested for use in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected Cdh1 in Western Blotting applications (Garzon, J., et. Al. (2017). Oncogene. 36(42):5808-5818). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged full-length recombinant mouse Fizzy-related protein homolog (Cdh1). |
| Clone | AR38.2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone AR38.2 detects Cdh1 (Fizzy-related protein homolog) in human and mouse cells. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~54 kDa observed; 54.69 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Cdh1 in HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |