| Description | |
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| Catalogue Number | MABT1341-25UG |
| Description | Anti-Lamin A/C Antibody, clone 4C11 |
| Alternate Names |
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| Background Information | Prelamin-A/C (UniProt: P02545) is encoded by the LMNA (also known as LMN1) gene (Gene ID: 4000) in human. It is cleaved into Lamin-A/C (also known as 70 kDa Lamin, Renal carcinoma antigen NY-REN-32). Lamins are components of the nuclear lamina that provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin A is initially synthesized as prelamin A that undergoes several modifications in the carboxyl terminal region that allow incorporation of prelamin A into the nuclear envelope and its subsequent processing into the mature lamin A. Cleavage of 15 residues (aa 647-662) by ZMPSTE24/FACE1 generates the final protein product. Unlike mature lamin A, prelamin A accumulates as discrete and localized foci at the nuclear periphery. Prelamin-A/C can accelerate smooth muscle cell senescence. It can act to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence. Mutations in LMNA gene are known to cause Emery-Dreifuss muscular dystrophy that is characterized by weakness and atrophy of muscle without involvement of the nervous system. Some mutations have also been linked to familial type of lipodystrophy characterized by the loss of subcutaneous adipose tissue in the lower parts of the body. (Ref.: Casasola, A., et Al. (2016). Nucleus 7(1); 84-102). |
| Product Information | |
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| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
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| Application | Anti-Lamin A/C, clone 4C11, Cat. No. MABT1341, is a highly specific mouse monoclonal antibody that targets Lamin A/C and has been tested for use in Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), and Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: A representative lot detected Lamin A/C in Western Blotting applications (Gesson, K., et. Al. (2016). Genome Res. 26(4):462-73; Roblek, M., et. Al. (2010). PLoS One. 5(5):e10604). DOT Blot Analysis: A representative lot detected Lamin A/C in DOT Blot applications (Roblek, M., et. Al. (2010). PLoS One. 5(5):e10604). Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lamin A/C in HeLa cells. Western Blotting Analysis: A representative lot detected Lamin A/C in WB used to demonstrate high titration and excellent properties of clone 4C11 (Courtesy of Marie lang, M.D., Stefan Schuchner, pH.D. and Egon Ogris, M.D., Medical University of Vienna, Austria). Immunofluorescence Analysis: A representative lot detected the Lamin A/C species at the nuclear lamina of HeLa cells (Courtesy of Marie Lang, M.D., Stefan Schuchner, pH.D. and Egon Ogris, M.D., Medical University of Vienna, Austria). Immunoprecipitation Analysis: A representative lot detected Lamin A/C in WB used to demonstrate high titration and excellent properties of clone 4C11 (Courtesy of Marie Lang, M.D., Stefan Schuchner, pH.D. and Egon Ogris, M.D., Medical University of Vienna, Austria). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Lamin A/C in Chromatin Immunoprecipitation applications (Gesson, K., et. Al. (2016). Genome Res. 26(4):462-73). Immunofluorescence Analysis: A representative lot detected Lamin A/C in Immunofluorescence applications (Roblek, M., et. Al. (2010). PLoS One. 5(5):e10604). Immunoprecipitation Analysis: A representative lot immunoprecipitated Lamin A/C in Immunoprecipitation applications (Gesson, K., et. Al. (2016). Genome Res. 26(4):462-73). |
| Biological Information | |
|---|---|
| Immunogen | Purified protein corresponding to the Ig-fold of human Lamin A/C. |
| Clone | 4C11 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 4C11 detects Lamin A/C at the nuclear lamina in multiple species. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse, Monkey, Rat, Syrian Hamster. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 74 and 65 kDa observed. 74.14 kDa and 65.14 kDa calculated for Lamin A and C, respectively. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected Lamin A/C in HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |