Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | ICC, IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT1350-100UG |
| Description | Anti-Autotaxin (ENPP2) Antibody, clone 7A5 |
| Alternate Names |
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| Background Information | Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (UniProt: Q13822; also known as EC: 3.1.4.39, E-NPP 2, Autotaxin, Extracellular lysophospholipase D, LysoPLD) is encoded by the ENPP2 (also known as ATX, PDNP2) gene (Gene ID: 5168) in human. Autotaxin is a secreted glycoprotein that is present in biological fluids. Its lysophospholipase D catalyzes the production of lysophosphatidic acid (LPA) in extracellular fluids. The level of LPA is shown to be markedly increased in the bronchoalveolar lavage fluid in asthma patients in response to allergens. Lysophosphatidylcholine is shown to be its major substrate. It can also act on sphingosylphosphphorylcholine to produce sphingosine-1-phosphate, a modulator of cell motility. Autotaxin is synthesized with a signal peptide (aa 1-27) and a propeptide (aa 28-35), which is cleaved off by furin in the mature form. Three isoforms of Autotaxin have been reported that are produced by alternative splicing. Isoform 1 is highly expressed in peripheral tissues and isoform 3 is more prominently expressed in the brain. Adipocytes are shown to express only isoform 1. Autotaxin levels are up-regulated in obese subjects with glucose intolerance and during adipogenesis. Autotaxin can also act as an angiogenic factor that stimulates migration of smooth muscle cells and microtubule formation and increased expression of Autotaxin has been reported in various types of cancers. (Ref.: Park, GY., et Al. (2013). Am. J. Respir. Crit. Care Med. 188(8); 928 940; Oikonomou, N., et Al. (2012). Am. J. Respir. Cell Mol. Biol. 47(5); 566-574). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Autotaxin (ENPP2), clone 7A5, Cat. No. MABT1350, is a mouse monoclonal antibody that detects Autotaxin (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected Autotaxin (ENPP2) in human kidney and human pancreas tissue sections. Immunohistochemistry Analysis: A representative lot detected Autotaxin (ENPP2) in Immunohistochemistry applications (Kaffe, E., et. Al. (2017). Hepatology. 65(4):1369-1383). Immunocytochemistry Analysis: A representative lot detected Autotaxin (ENPP2) in Immunocytochemistry applications (Kaffe, E., et. Al. (2017). Hepatology. 65(4):1369-1383). Western Blotting Analysis: A representative lot detected Autotaxin (ENPP2) in Western Blotting applications (Kaffe, E., et. Al. (2017). Hepatology. 65(4):1369-1383). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant fragment (without the first 28 amino acids) from human Autotaxin. |
| Clone | 7A5 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 7A5 detects human and murine autotaxin. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~100 kDa observed; 98.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting with recombinant human autotaxin. Western Blotting Analysis: 0.2 µg/mL of this antibody detected recombinant human autotaxin. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |