Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IF, Inhibition, WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT1357-25UL |
| Description | Anti-IZUMO1 Antibody, clone Mab120 |
| Alternate Names |
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| Background Information | Izumo sperm-egg fusion protein 1 (UniProt: Q9D9J7; also known as Oocyte binding/fusion factor, OBF, Sperm-specific protein izumo) is encoded by the Izumo1 gene (Gene ID: 73456) in murine species. OBF is a single-pass type I membrane protein that is detected on sperm surface after the acrosome reaction. It is an essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. It can be present as a monomer, homodimer, or homooligomer. It is synthesized with a signal peptide (aa 1-21), which is cleaved off in the mature form, which has an extracellular domain (aa 22-319), a short transmembrane domain (aa 320-340), and a cytoplasmic domain (aa 341-397). The extracellular domain assumes a distinct boomerang shape when not bound to IZUMO1R/JUNO. Its interaction with IZUMO1R/JUNO triggers a conformation change, SO that the IZUMO1 extracellular domain assumes an upright conformation. A region comprising of 13 amino acids (148-160) is important for its interaction with IZUMO1R. Mab120 is a fusion-inhibiting antibody that inhibits fertilization. It binds to a region designated as putative functional fragment (aa 5-113). (Ref.: Inoue, N et Al. (2013). Development. 140(15); 3221-3229). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2a in PBS without azide. |
| Applications | |
|---|---|
| Application | Anti-IZUMO1, clone Mab120, Cat. No. MABT1357, is a rat monoclonal antibody that detects Izumo sperm-egg fusion protein 1 and has been tested for use in Immunofluorescence, Inhibition assay, and Western Blotting. |
| Key Applications |
|
| Application Notes | Inhibition Analysis: A representative lot inhibited fertilization in an in vitro system. (Inoue, N., et. Al. (2013). Development. 140(15):3221-9). Western Blotting Analysis: A representative lot detected IZUMO1 in Western Blotting applications (Inoue, N., et. Al. (2013). Development. 140(15):3221-9). Immunofluorescence Analysis: A representative lot detected IZUMO1 in Immunofluorescence applications (Inoue, N., et. Al. (2013). Development. 140(15):3221-9). |
| Biological Information | |
|---|---|
| Immunogen | Mouse spermatozoa. |
| Clone | Mab120 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone Mab120 is a rat monoclonal antibody that detects Izumo sperm-egg fusion protein 1 (IZUMO1) in murine species. It targets an epitope with in the N-terminal putative functional fragment. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~56 kDa observed; 44.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse testis tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected IZUMO1 in mouse testis tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |