Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H | ICC, IH(P), IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT1363-25UL |
| Description | Anti-GM130 Antibody, clone 4A3 |
| Alternate Names |
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| Background Information | Golgin subfamily A member 2 (UniProt: Q62839; also known as 130 kDa cis-Golgi matrix protein, GM130) is encoded by the Golga2 gene (Gene ID: 64528) in rat. GM130 is a peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton to maintain the structure of the Golgi apparatus, and as a vesicle tether that facilitates vesicle fusion to the Golgi membrane. GM130 is a homotetramer with a flexible rod-like structure. It has a nuclear localization sequence (aa 26-49) that mediates interaction with importin-alpha and recruits it to the Golgi membrane and liberates TPX2. GM130 promotes the mitotic spindle pole assembly by activating TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle. Phosphorylation of GM130 at Ser37 by CDK1 at the onset of mitosis inhibits its interaction with p115/USO1, which prevents its tethering of COPI vesicles and thereby inhibits transport through the Golgi apparatus during mitosis. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-GM130, clone 4A3, Cat. No. MABT1363, is a mouse monoclonal antibody that detects Golgin subfamily A member 2. (GM130) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected GM130 in human liver cancer tissue. Western Blotting Analysis: A representative lot detected GM130 in Western Blotting applications (Diao, A., et. al. (2003) J Cell Biol. 160(2):201-12). Immunocytochemistry Analysis: A representative lot detected GM130 in Immunocytochemistry applications (Seemann, J., et. al. (2002). Science. 295(5556):848-51; Diao, A., et. al. (2003) J Cell Biol. 160(2):201-12). Immunohistochemistry Analysis: A representative lot detected GM130 in Immunohistochemistry applications (Seemann, J., et. al. (2002). Science. 295(5556):848-51). Immunoprecipitation Analysis: A representative lot detected GM130 in Immunoprecipitation applications (Diao, A., et. al. (2003) J Cell Biol. 160(2):201-12; Diao, A., et. al. (2008) J Cell Biol. 283(11):6957-67). |
| Biological Information | |
|---|---|
| Immunogen | A full length recombinant human Golgin subfamily A member 2 (GM130). |
| Clone | 4A3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 4A3 detected Golgin subfamily A member 2 (GM130) in human liver cancer and human intestine sections. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 112.87 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human small intestine tissue. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected GM130 in human small intestine tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |