| Description | |
|---|---|
| Catalogue Number | MABT1365-25UL |
| Description | Anti-Tropomyosin-1 Antibody, clone 7F8B8 |
| Alternate Names |
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| Background Information | Tropomyosin alpha-1 chain (UniProt: P09493; also known as Alpha-tropomyosin, Tropomyosin-1) is encoded by the TPM1 (also known as C15orf13, TMSA) gene (Gene ID: 7168) in human. Tropomyosin-1 is a cytoskeletal protein that associates with F-actin stress fibers. It binds to actin filaments in muscle and non-muscle cells and plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. It stabilizes actin filaments and regulates the interaction of actin filament with other actin-binding proteins. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Tropomyosin-1 is a homodimeric protein, but can form heterodimer of an alpha (TPM1, TPM3, or TPM4) and a beta (TPM2) chain. Mutations in TPM1 gene have been linked to cardiomyopathy characterized by ventricular hypertrophy and ventricular dilation and impaired systolic function that results in congestive heart failure and arrhythmia. Ten different isoforms of Tropomyosin-1 have been described that are produced by alternative splicing. |
| Product Information | |
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| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
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| Application | Anti-Tropomyosin-1, clone 7F8B8, Cat. No. MABT1365, is a rat monoclonal antibody that detects Tropomyosin-1 and has been tested for use in Immunocytochemistry and Western Blotting. |
| Key Applications |
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| Application Notes | Immunocytochemistry Analysis: A representative lot detected Tropomyosin-1 in Immunocytochemistry applications (Brayford, S., et. Al. (2016). Curr Biol. 26(10):1312-8). |
| Biological Information | |
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| Immunogen | KLH-conjugated linear peptides corresponding to 24 amino acids from the N-terminal region of isoform 5 and 4 amino acids from the N-terminal region of isoform 2 of human tropomyosin-1. |
| Epitope | N-terminus |
| Clone | 7F8B8 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 7F8B8 detects human and murine Tropomyosin-1. It targets an epitope with in the N-terminal region. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~30 kDa observed; 28.38 kDa for isoform 5. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting in mouse embryonic fibroblast (MEF) lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Tropomyosin-1 in mouse embryonic fibroblast (MEF) lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 µL |