| Description | |
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| Catalogue Number | MABT1366-25UG |
| Description | Anti-CRB3A Antibody, clone 1E6 |
| Alternate Names |
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| Background Information | Protein crumbs homolog 3 (UniProt: Q9BUF7) is encoded by the CRB3 (also known as UNQ588/PRO1158) gene (Gene ID: 92359) in human. Mammalian cells contain three Crb orthologs. CRB1 is primarily expressed in the brain, cornea, and retina. CRB2 is expressed in lung, kidney, retina, colon and mammary glands. It is also expressed in at moderate levels in Expressed at moderate levels in liver, spleen, pancreas, placenta, and prostate tissue. CRB3 is present in epithelial tissues and is present in two isoforms, CRB3A and CRB3B. CRB3 is a single-pass type I membrane protein that is reported to control epithelial cell polarization. CRB3A is apically localized and promotes apical membrane growth and is and is essential for the formation of tight junctions in epithelial cells. It also prevents the epithelial-to-mesenchymal transition (EMT), which is known to contribute to tumor progression. CRB3 is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off. The mature form contains an extracellular domain (aa 27-59), a transmembrane domain (aa 60-80), and a cytoplasmic tail (aa 81-120), which contains a PDZ-domain binding motif (aa 117-120) that interacts with the Maguk protein, PALS1, which recruits PALS1-associated tight junction (PATJ) protein into the CRB3A complex. Both PALS1 and PATJ play a critical role in epithelial cell polarity and tight junction formation. CRB3A is shown to control and properly localize RhoA signaling that is required for CRB3A-dependent reorganization of the actin cytoskeleton. (Ref.: Loie, E., et Al (2015). Mol. Cell Biol. 35(19); 3423-3435). |
| Product Information | |
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| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
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| Application | Anti-CRB3A, clone 1E6, Cat. No. MABT1366, is a rat monoclonal antibody that detects Protein crumbs homolog 3 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: 2 µg/mL from a representative lot detected CRB3A in Caco2 cell lysates over-expressing Crumbs 3A (positive) versus lysates from Caco2 cells silenced with siRNA (negative). Western Blotting Analysis: A representative lot detected CRB3A in Western Blotting applications (Loie, E., et. Al. (2015). Mol Cell Biol. 35(19):3423-35). |
| Biological Information | |
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| Immunogen | KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Protein crumbs homolog 3 (CRB3). |
| Epitope | C-terminus |
| Clone | 1E6 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 1E6 is a rat monoclonal antibody that detects protein crumbs homolog 3 in human cells. it targets an epitope with in 15 amino acids from the C-terminal region. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Drosophila, Mouse based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~35 kDa observed; 12.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected CRB3A in human kidney tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |