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Millipore Anti-Claudin-4 Antibody, Clone 10b4.3 Is An Antibody Against Claudin-4 For Use In Western Blotting & Ihc (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
Williams-Beuren syndrome chromosomal region 8 protein
Background Information
Claudin 4, also known as Clostridium perfringens enterotoxin receptor, or CPE-R, or CPE-receptor or Williams-Beuren syndrome chromosomal region 8 protein, and encoded by the gene CLDN4/CPER/CPETR1/WBSCR8, plays a critical role in tight junction specific obliteration of intercellular space. Claudin 4 directly interacts with several important tight junction associated proteins including ZO-1,2,3 and EPHA2 proteins, and through these interactions, Claudin 4 regulates tight junction formation. Claudin 4 is localized to cell junctions and membranes at tight junctions. Claudin 4 is expressed in most tissues particularly in intestine, liver, and pancreas. Defects in Claudin 4 may be associated with Williams Beuren syndrome genetic deletion syndrome. Claudin 4 is also over expressed in many cancers and thus is a target of anti-cancer research using C. perfringens enterotoxin.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Claudin-4 Antibody, clone 10B4.3 is an antibody against Claudin-4 for use in western blotting & IHC (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Claudin-4 in 10 µg of MCF-7 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Claudin-4 in human ileum and human tonsil tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Claudin-4.
Epitope
Cytoplasmic domain
Clone
10B4.3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001296
Gene Symbol
CLDN4
CPER
CPETR1
WBSCR8
Purification Method
Protein G Purified
UniProt Number
O14493
Molecular Weight
~ 22 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Claudin-4 in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
