| Description | |
|---|---|
| Catalogue Number | MABT1491-25UG |
| Description | Anti-ZNF175 (OTK18) Antibody, clone 211−3.1−1 |
| Alternate Names |
|
| Background Information | Zinc finger protein 175 (UniProt: Q9Y473; also known as Zinc finger protein OTK18) is encoded by the ZNF175 gene (Gene ID: 7728) in human. ZNF175 is zinc finger protein that contains 13 C2H2 type zinc fingers and is shown to be ubiquitous in its expression. ZNF175 displays antiviral activity and it is produced during HIV-1 infection of mononuclear phagocytes in human and interferes with HIV-1 replication by suppressing Tat-induced viral LTR promoter activity. It down-regulates the expression of several chemokine receptors and plays a role in the interaction of viral and host cell factors leading to HIV-1 associated dementia and HIV-1 encephalitis. ZNF175 contains a Krüppel associated box (KBAB; aa 26-89) domain that is responsible for its repressive activity. Plasma levels of ZNF175 are reported to correlate with CD8 count and HIV status. (Ref.: Buescher, J., et Al. (2008). J Neuroimmune Pharmacol. 3(4); 230-235). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-OTK18(ZNF175) clone 211 3.1 1, Cat. No. MABT1491, is a mouse monoclonal antibody that detects Zinc finger protein 175 (OTK18) and has been tested for use in ELISA and Immunocytochemistry. |
| Key Applications |
|
| Application Notes | ELISA Analysis: A representative lot detected OTK18(ZNF175) in ELISA applications (Buescher, J.L., et. Al. (2008). J Neuroimmune Pharmacol. 3(4):230-5). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant fragment corresponding to the first 178 amino acids from the N-terminal region of human Zinc finger protein 175. |
| Epitope | N-terminus |
| Clone | 211−3.1−1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 211 3.1 1 specifically detects human Zinc finger protein 175. It targets an epitope with in the first 178 amino acids from the N-terminal region. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein g purified |
| UniProt Number |
|
| Molecular Weight | 81.61 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in HeLa cells. Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected OTK18(ZNF175) in HeLa cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |