Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, ICC, IHC, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT1495-100UG |
| Description | Anti-PD2 (PAF1) Antibody, clone F169-3B2 |
| Alternate Names |
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| Background Information | Pancreatic differentiation protein 2 (UniProt: Q8N7H5; also known as RNA polymerase II-associated factor 1 homolog, PD2, hPAF1) is encoded by the PAF1 (also known as PD2) gene (Gene ID: 54623) in human. PD2 is a nuclear protein that serves as a subunit of the RNA polymerase II associated factor (PAF) complex. It has multiple functions during transcription by RNA polymerase II. It has been implicated in regulation of development and maintenance of embryonic stem cell pluripotency. It associates with RNA polymerase II through interaction with POLR2A C-terminal domain non-phosphorylated and serine 2 and serine 5 phosphorylated forms. It is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. Three isoforms of PD2 have been described that are produced by alternative splicing. PD2 has oncogenic property and is shown to be overexpressed in pancreatic cancer cells. In normal murine pancreas, its expression is restricted to acinar cells, however, its expression is increased in the ductal cells of mouse model of pancreatic cancer with advancing age. (Ref.: Dey, P., et Al (2014). Oncotarget, 5(12); 4480-4491). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS without azide. |
| Applications | |
|---|---|
| Application | Anti-PD2 (PAF1), clone F169-3B2, Cat. No. MABT1495, is mouse monoclonal antibody that detects Pancreatic differentiation protein 2 and has been tested for use in ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot detected PD2 (PAF1) in Immunocytochemistry applications (Dey, P., et. Al. (2014). Oncotarget. 5(12):4480-91; Moniaux, N., et. Al. (2006). Oncogene. 25(23):3247-57). Immunoprecipitation Analysis: A representative lot immunoprecipitated PD2 (PAF1) in Immunoprecipitation applications (Moniaux, N., et. Al. (2006). Oncogene. 25(23):3247-57). ELISA Analysis: A representative lot detected PD2 (PAF1) in ELISA applications (Moniaux, N., et. Al. (2006). Oncogene. 25(23):3247-57). Immunohistochemistry Analysis: A representative lot detected PD2 (PAF1) in Immunohistochemistry applications (Dey, P., et. Al. (2014). Oncotarget. 5(12):4480-91). Western Blotting Analysis: A representative lot detected PD2 (PAF1) in Western Blotting applications (Moniaux, N., et. Al. (2006). Oncogene. 25(23):3247-57). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human Pancreatic differentiation protein 2 (PD2). |
| Clone | F169-3B2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone F169-3B2 detects Pancreatic differentiation protein 2 (PD2) in human. It targets an epitope with in 22 amino acids from the C-terminal half. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 59.98 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in Panc1 cells. Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected PD2 (PAF1) in Panc1 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |