Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, FC, IF, IH(P), IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT1502-25UG |
| Description | Anti-Integrin beta-1 Antibody, clone 102DF5 |
| Alternate Names |
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| Background Information | Integrin beta-1 (UniProt: P05556; also known as Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12) gene (Gene ID: 3688) in human. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. Integrin beta-1 is a single-pass type I membrane protein that can associate with one of the multiple alpha subunits to serve as a receptor for fibronectin and other extracellular matrix proteins. Integrin beta-1 integrin recognizes the sequence R-g-D in a wide array of ligands. Five isoforms of integrin beta-1 have been reported that are produced by alternative splicing. Integrin beta-1 is synthesized with a signal peptide (aa 1-20) that is subsequently cleaved off. The mature form has an extracellular domain (aa 21-728), a helical domain (aa 729-751) and a cytoplasmic region (aa 752-798). Isoform 1 of integrin beta-1 is widely expressed and other isoforms are generally co-expressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4 is selectively expressed in peripheral T-cells and isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle). Isoform 2 is reported to interfere with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Integrin beta-1, clone 102DF5, Cat. No. MABT1502, is a mouse monoclonal antibody that detects Integrin beta-1 and has been tested for use in ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A representative lot detected Integrin beta-1 in Flow Cytometry applications (Belkin, A.M., et. Al. (1997). J Cell Biol. 139(6):1583-95). Immunofluorescence Analysis: A representative lot detected Integrin beta-1 in Immunofluorescence applications (Cartier-Michaud, A., et. Al. (2012). PLoS One. 7(2):e32204; Balzac, F., et. Al. (1993). J Cell Biol. 121(1):171-8; Lin, Y.N., et. Al. (2015). Oncotarget. 6(21):18577-89). Western Blotting Analysis: A representative lot detected Integrin beta-1 in Western Blotting applications (Balzac, F., et. Al. (1993). J Cell Biol. 121(1):171-8). ELISA Analysis: A representative lot detected Integrin beta-1 in ELISA applications (Jovanovic, M., et. Al. (2010). Act Histochem. 112(1):34-41). Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin beta-1 in Immunoprecipitation applications (Balzac, F., et. Al. (1993). J Cell Biol. 121(1):171-8). |
| Biological Information | |
|---|---|
| Immunogen | Tissue extract from human myometrium. |
| Clone | 102DF5 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 102DF5 specifically detects integrin beta-1 subunit in human cells. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~150 kDa observed; 88.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in human uterus and human kidney tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected Integrin beta-1 in human uterus and human kidney tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |