Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, Porcine | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT1504-100UG |
| Description | Anti-Calponin Antibody, clone CALP |
| Alternate Names |
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| Background Information | Calponin-1 (UniProt: P51911; also known as Basic calponin, Calponin H1 smooth muscle) is encoded by the CNN1 gene (Gene ID:1264) in human. Calponin is an actin filament-associated regulatory protein that is expressed in smooth muscle and non-muscle cells of vertebrates. Three isoforms of Calponin have been reported that inhibit actin-activated myosin ATPase activity and stabilize the actin cytoskeleton. Calponin 1 is specifically expressed in smooth muscle cells is involved in fine-tuning of smooth muscle contractility. Calponin-2 is found in multiple cell types, including developing smooth muscles and Calponin-3 is found in the neurons, astrocytes and glial cells. Calponin-1 can bind to actin, calmodulin, troponin C, and tropomyosin. Calponin-1 (and other calponins) has a calponin homology (CH) domain at its N-terminal (aa 28-131) that can function either as a part of actin-binding site or serve as a regulatory structure for actin-binding activity. It also has three calponin-like domains (aa 164-189; 204-229; and 243-268) next to the C-terminal region that are conserved in all calponins. The C-terminal segment varies among different calponins and has differentiated effects on weakening the binding of calponin to F-actin. Deletion of the C-terminal tail segment is shown to enhance the actin-binding and bundling activities of calponins. (Ref.: Liu, R., and Jin, J-P (2016). Gene 585(1); 143-153). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Calponin, clone CALP , Cat. No. MABT1504, is a mouse monoclonal antibody that detects Calponin-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Calponin in human uterus and human prostate tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | Crude human uterine muscle extract. |
| Clone | CALP or N3/16 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone CALP specifically detects human Calponin-1. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human, Bovine, Porcine. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~36 kDa observed; 33.17 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human aortic smooth muscle tissue lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Calponin in human aortic smooth muscle tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |