Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, ICC, IF, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT1506-100UG |
| Description | Anti-Desmoglein 3 Antibody, clone 5H10 |
| Alternate Names |
|
| Background Information | Desmoglein-3 (UniProt: P32926; also known as 130 kDa pemphigus vulgaris antigen, PVA, Cadherin family member 6) is encoded by the DSG3 (also known as CDHF6) gene (Gene ID: 1830) in human. Desmogleins are protein substances located in and on the surface of keratinocytes. They are present in specialized cell-cell adhesion structures enriched in tissues that undergo substantial mechanical stress. These proteins have been shown to be a critical factor in cell-to-cell adhesion. Desmoglein-3 is a single-pass type I membrane protein that is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It is synthesized with a signal peptide (aa 1-23) and a propeptide (aa 24-49) that are cleaved off in the mature form. It contains an extracellular domain (aa 50-615), a transmembrane domain (aa 616-640), and a cytoplasmic domain (aa 641-999). Desmoglein-3 has four cadherin domains and two desmoglein repeats. This antibody (clone 5H10) recognized the extracellular domain of desmoglein-3. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Desmoglein 3, clone 5H10, Cat. No. MABT1506, is a mouse monoclonal antibody that detects Desmoglein-3 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot detected Desmoglein 3 in Immunoprecipitation applications (Wahl, J.K. 3rd, et. al. (2002). Hybrid Hybridomics. 21(1):37-44). Flow Cytometry Analysis: A representative lot detected Desmoglein 3 in Flow Cytometry applications (Wan, H., et. al. (2007). Stem Cells. 25(5):1286-97). Western Blotting Analysis: A representative lot detected Desmoglein 3 in Western Blotting applications (Wahl, J.K. 3rd, et. al. (2002). Hybrid Hybridomics. 21(1):37-44). Immunocytochemistry Analysis: A representative lot detected Desmoglein 3 in Immunocytochemistry applications (Wahl, J.K. 3rd, et. al. (2002). Hybrid Hybridomics. 21(1):37-44). Immunofluorescence Analysis: A representative lot detected Desmoglein 3 in Immunofluorescnece applications (Wan, H., et. al. (2007). Stem Cells. 25(5):1286-97). |
| Biological Information | |
|---|---|
| Immunogen | Extracellular domain of Desmoglein 3 |
| Epitope | extracellular domain |
| Clone | 5H10 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 5H10 detects Desmoglein-3 in human cells. It targets an epitope with in the extracellular domain. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G purified |
| UniProt Number |
|
| Molecular Weight | ~125 kDa observed; 107.53 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HaCaT cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Desmoglein 3 in HaCaT cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |