Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, H | ICC, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT1508-25UG |
| Description | Anti-Alpha-Catenin Antibody, clone 1G5 |
| Alternate Names |
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| Background Information | Catenin alpha 1 (UniProt: E1C7M7) is encoded by the CTNNA1 gene in chicken. Catenins are a large family of cell-cell adhesion molecules that are involved in development and in epithelial cell polarity. Catenins are reported to associate with both E- and N-cadherins. The association of catenins to cadherins creates a complex that is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Alpha-Catenin is considered as an important regulator of actin dynamics in cell-cell adhesion and it forms a heterodimer with beta-Catenin and links the cadherins to actin. Unlike other catenins, alpha-catenin does not bind to cadherins directly, but is linked to the adhesive complexes of AJs via association with -catenin. Alpha-catenin can also bind and bundle actin filaments and can interact with a number of protein regulators of actin filament turnover. Usually cells contain a significant amount of cadherin-free alpha-catenin and this pool of free alpha-catenin can impact actin dynamics, such as limiting Arp2/3-based actin polymerization and severing by cofilin that ultimately promotes the assembly and stabilization of unbranched filament bundles. Alpha-catenin expression in a colon cancer cell lines has been correlated with increased Tcf-dependent transcription and is shown to inhibit beta-catenin signaling in the nucleus by interfering with the formation of a beta-catenin-Tcf-DNA complex. (Ref.: Giannini, Al et Al. (2000). J. Bol. Chem. 275(29); 21883-8). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Alpha-Catenin, clone 1G5, Cat. No. MABT1508, is a mouse monoclonal antibody that detects Catenin alpha 1 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot immunoprecipitated Alpha-Catenin in Immunoprecipitation applications (Knudsen, K.A., et. Al. (1995). J Cell Biol. 130(1):67-77; Johnson, K.R., et. Al. (1993). Exp Cell Res. 207(2):252-60). Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Alpha-Catenin in A431 cell line. Western Blotting Analysis: A representative lot detected Alpha-Catenin in Western Blotting applications (Nieman, M.T., et. Al. (1999). J Cell Sci. 112 ( Pt 10):1621-32; Knudsen, K.A., et. Al. (1995). J Cell Biol. 130(1):67-77; Johnson, K.R., et. Al. (1993). Exp Cell Res. 207(2):252-60). Immunocytochemistry Analysis: A representative lot detected Alpha-Catenin in Immunocytochemistry applications (Johnson, K.R., et. Al. (1993). Exp Cell Res. 207(2):252-60). |
| Biological Information | |
|---|---|
| Immunogen | MBP-conjugated full-length chicken recombinant Catenin alpha 1. |
| Clone | 1G5 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 1G5 specifically detects alpha-catenin 1 in human and chicken cells. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Chicken, Human. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~108 kDa observed; 59.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Alpha-Catenin in A431 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |