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Millipore Detect Versican Using This Mouse Monoclonal Antibody, Anti-Versican Antibody, Clone 8d8.1 Validated For Use In Ihc & Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan. Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest.
Product Information
Format
Purified
Control
Human thalamus tissue lysate
Presentation
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Versican using this mouse monoclonal antibody, Anti-Versican Antibody, clone 8D8.1 validated for use in IHC & western blotting.
Key Applications
Immunohistochemistry
Western Blotting
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Versican in human cerebellum, and mouse brain and embryonic skeletal muscle.
Western Blotting Analysis: 0.2 µg/mL from a representative lot detected Versican in mouse leg muscle tissue lysate.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of human Veriscan.
Epitope
C-terminus
Clone
8D8.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes the C-terminus of Veriscan.
Isotype
IgMκ
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_00119808
Gene Symbol
VACN
CSPG2
UniProt Number
P13611
Molecular Weight
372 kDa calculated. Uniprot describes three isoforms at 265 kDa, 182 kDa, and 74 kDa. Versican is known to be secreted following valvular interstitual cell (VIC) injury and repair (Carthy, J., et al. (2012). Cardiovac. Pathol. 21(2):74-82.).
Product Usage Statements
Quality Assurance
Evaluated by Immunohistochemistry in human thalamus tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Versican in human thalamus tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
