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Millipore Anti-Cytokeratin 8, Clone Troma-1 Antibody, Cat. No. Mabt329-25ug Is Validated For Use In Western Blotting, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, - Mill (Additional S&H or Hazmat Fees May Apply)
Keratin, type II cytoskeletal 8 (UniProt: P11679; also known as Cytokeratin endo A, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8) is encoded by the Krt8 (also known as Krt2-8) gene (Gene ID: 16691) in murine species. Cytokeratins belong to a diverse group of intermediate filaments that participate in differentiation and maintain the structural integrity of epithelial cells. Cytokeratins share a common structural organization: a central a-helical rod domain flanked by head and tail domains. An important unique feature of cytokeratins is their epithelial cell-type-specific expression. CK8 is a heterotetramer of two type I and two type II keratins. It is expressed in abundance in the epithelia of colon, bladder, ileum, and stomach. CK8 together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. CK8 expression has been correlated with malignancy in leukoplakia and carcinomas of the head and neck and its expression has been observed in all non-small-cell lung cancers.
Product Information
Format
Purified
Presentation
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Cytokeratin 8, clone TROMA-1 Antibody, Cat. No. MABT329-25UG is validated for use in Western Blotting, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Immunofluorescence and Radioimmunoassay.
Key Applications
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Immunofluorescence
Radioimmunoassay
Application Notes
Immunocytochemistry Analysis: A representative lot detected Cytokeratin 8 in Immunocytochemistry applications (ODriscoll, C.M., et. al. (2013). Biochim Biophys Acta. 1833(3):460-7; Wang, X., et. al. (2004). J Cell Sci. 117(Pt 1):53-62).
Biological Information
Immunogen
Intermediate filaments extracted from TDM1 Trophoblastoma cells.
Concentration
Please refer to lot specific datasheet.
Host
Rat
Specificity
Clone TROMA-1 detects cytokeratin-8 in human and murine cells.
Isotype
IgG2a
Species Reactivity
Human
Mouse
Species Reactivity Note
Human, Mouse.
Antibody Type
Monoclonal Antibody
Gene Symbol
Krt8
Krt2-8
Purification Method
Protein G purified
UniProt Number
P11679
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
