The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Detect Myl3 Using This Anti-Myl3, Clone 7c1 Antibody Validated For Use In Western Blotting & Ihc. - Mill (Additional S&H or Hazmat Fees May Apply)
Myosin light chain 3 (MYL3), also known as Cardiac myosin light chain 1 (CMLC1) or Myosin light chain 1 slow-twitch muscle B/ventricular isoform (MLC1SB) or Ventricular/slow twitch myosin alkali light chain (VLC1) functions as a regulatory light chain of myosin. Myosins are a large superfamily of motor proteins that move along actin filaments, while hydrolyzing ATP. Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains and four light chains. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Human myosin light chain has clinical application as a cardiac marker. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy.
Product Information
Format
Ascites
Presentation
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
Detect MYL3 using this Anti-MYL3, clone 7C1 Antibody validated for use in western blotting & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected MYL3 in human skeletal muscle and cardiac muscle tissues.
Biological Information
Immunogen
Purified recombinant fragment of human MYL3 expressed in E. Coli
Clone
7C1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1
Species Reactivity
Rat
Antibody Type
Monoclonal Antibody
Gene Symbol
MYL3
Purification Method
Unpurified
UniProt Number
P08590
Molecular Weight
~25 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in rat fetal heart tissue lysate.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected MYL3 in rat fetal heart tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
