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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IP, IHC, ICC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT51 |
| Description | Anti-Galectin-3 Antibody, clone M3/38 |
| Alternate Names |
|
| Background Information | Galectin-3 belongs to the galectin gene family defined by the specificity of a carbohydrate recognition domain (CRD) for Gal/Lac and expression is usually elevated in a wide range of neoplastic cell types. The galectin CRD consists of 135 amino acids. Galectin-3 has been shown to effect tumor development and progression. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Galectin-3 Antibody, clone M3/38 is an antibody against Galectin-3 for use in WB, IP, IH & IC. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 1:50,000 dilution from a previous lot detected Galectin-3 in 10 µg of NIH/3T3 cell lysate. Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Springer, T., et al. (1981). THE JOURNAL OF BIOLOGICACHLE MISTRY. 256(8):3833-3839.) Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH. (Gasbarri, A., et al. (1999). Journal of Clinical Oncology. 17(11):3494-3502.) Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Gasbarri, A., et al. (1999). Journal of Clinical Oncology. 17(11):3494-3502.) |
| Biological Information | |
|---|---|
| Immunogen | Plasma membrane glycoproteins corresponding to mouse Galectin-3. |
| Clone | M3/38 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). SUBUNIT STRUCTURE: Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA. SUBCELLULAR LOCATION: Nucleus. Note: Cytoplasmic in adenomas and carcinomas (By similarity). May be secreted by a non-classical secretory pathway and associate with the cell surface. TISSUE SPECIFICTY: The highest levels are found in activated macrophages. SEQUENCE SIMILARITIES: Contains 1 galectin domain. |
| Molecular Weight | ~28 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 1:50,000 dilution of this antibody detected Galectin-3 in 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |