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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT63 |
| Description | Anti-Nectin-3/PVRL3 (CD113) Antibody, clone N3.12.4 |
| Alternate Names |
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| Background Information | Nectin-3, also known as Poliovirus receptor-related protein 3 (PVRL3) and PRR3, is thought to work with cadherin to form cell-cell adherens junctions. It is a transmembrane protein that is commonly found in the testis and in placental tissue. Nectin-3 colocalizes with CD155, the cognate receptor for poliovirus, to cell-cell junctions. Nectin-3s involvement in down-regulation PVR from the cell surface leads to a reduction in cell motility and proliferation. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Nectin-3/PVRL3 (CD113) Antibody, clone N3.12.4 detects level of Nectin-3/PVRL3 (CD113) & has been published & validated for use in FC. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Full length recombinant protein corresponding to human Nectin-3. |
| Clone | N3.12.4 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Nectins (e.g., PVRL1; MIM 600644) are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin (see MIM 192090)-catenin (see MIM 116805) system in epithelial cells. [supplied by OMIM]. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. SUBUNIT STRUCTURE: Cis- and trans-homodimer. Can form trans-heterodimers with PVRL1/nectin-1, PVRL2/nectin-2, PVR, IGSF4B/Necl-1 and with IGSF4. Interacts with MLLT4/afadin. Binds with low affinity to TIGIT. SUBCELLULAR LOCATION: Cell membrane; Single-pass membrane protein Potential. TISSUE SPECIFICTY: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines. SEQUENCE SIMILARITIES: Belongs to the nectin family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SEQUENCE CAUTION: The sequence AAH17572.1 differs from that shown. Reason: Erroneous initiation. |
| Molecular Weight | 61 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in U251 cells. Flow Cytometry Analysis: 0.1 µg of this antibody detected Nectin-3 in U251 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |