Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC, IP, Inhibition | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT820 |
| Description | Anti-Integrin alpha 5 (CD49e) Antibody, clone mAb16 |
| Alternate Names |
|
| Background Information | Integrin alpha-5 (UniProt: P08648; also known as CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e) is encoded by the ITGA5 (also known as FNRA) gene (Gene ID: 3678) in human. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. Integrin alpha-5 is a single-pass type I membrane protein is expressed on multiple cell types. It contains four calcium binding sites (aa 480-488; 334-342; 401-409; and 465-473). Structurally, it consists of an extracellular domain (aa 42-995), a transmembrane domain (aa 996-1021), and a cytoplasmic domain (aa 1022-1049). When associated with beta 1 subunit it can serve as a receptor for fibronectin and fibrinogen. It recognizes the sequence R-g-D in its ligands. Integrin alpha-5 is also expressed in articular cartilage and plays a role in synovial joint development and in the regulation of chondrocyte survival and matrix degradation in articular cartilage. Membrane fractions obtained from fibroblasts of diabetic animals display significant reduction in integrin alpha-5 levels. (Ref.: Almeida, ME et Al., (2016). Mol. Cell. Biochem. 421(1-2); 19-28). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2a in PBS without azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Integrin alpha5 (CD49e), clone mAb16, Cat. No. MABT820, is a rat monoclonal antibody that detects Integrin alpha5 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Inhibition assays. |
| Key Applications |
|
| Application Notes | Inhibition Analysis: 10 ug/mL from a representative lot prevented HT-1080 cells from adhering to fibronectin coated plates (Courtesy of Dr. Kenneth M. Yamada, M.D., pH.D., NIDCR, National Institutes of Health, Bethesda, MD USA). Inhibition Analysis: A representative lot inhibited WI38 fibroblast attachment to fibronectin (Akiyama, S.K., et. Al. (1989). J Cell Biol. 109(2):863-75). Inhibition Analysis: A representative lot inhibited A375-SM cell spreading. (Humphries, J.D., et. Al. (2005). J Biol Chem. 280(11):10234-43). Immunocytochemistry Analysis: A representative lot detected Integrin alpha5 (CD49e) in Immunocytochemistry applications (Akiyama, S.K., et. Al. (1989). J Cell Biol. 109(2):863-75). Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin alpha5 (CD49e) in Immunoprecipitation applications (Akiyama, S.K., et. Al. (1989). J Cell Biol. 109(2):863-75). |
| Biological Information | |
|---|---|
| Immunogen | Fibronectin receptor purified from human placenta. |
| Clone | mAb16 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone mAb16 is a rat monoclonal antibody that specifically detects Integrin alpha5 in human cells. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein g purified |
| UniProt Number |
|
| Molecular Weight | 114.54 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in HFF cells. Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected Integrin alpha5 (CD49e) in human Foreskin Fibroblast (HFF) cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |