| Description | |
|---|---|
| Catalogue Number | MABT846-25UG |
| Description | Anti-MYH-1 Antibody, clone 6F12H3 |
| Alternate Names |
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| Background Information | Myosin-1 (UniProt: Q5SX40; also known as Myosin heavy chain 1, Myosin heavy chain 2x, MyHC-2x, Myosin heavy chain, skeletal muscle, adult 1) is encoded by the Myh1 gene (Gene ID: 17879) in murine species. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular sub-fragments (S1) and 1 rod-shaped sub-fragment (S2). In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and non-muscle cells. These isoforms show expression that is spatially and temporally regulated during development. Myosin-1 contains an N-terminal SH3-like domain (aa 33-82), a myosin motor domain (aa 86-785), and an IQ domain (aa 788-817). It also contains 2 actin binding regions (aa 662-684 and 764-778). |
| Product Information | |
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| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
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| Application | Anti-MYH-1, clone 6F12H3, Cat. No. MABT846, is a rat monoclonal antibody that detects Myosin-1 and has been tested for use in Immunofluorescence. Immunohistochemistry, and Western Blotting. |
| Key Applications |
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| Application Notes | Immunohistochemistry Analysis: A 1:25 dilution from a representative lot detected MYH-1 in mouse heart and mouse skeletal muscle tissue. Immunofluorescence Analysis: A representative lot detected MYH-1 in Immunofluorescence applications (Sawano, S., et. Al. (2016). PLoS One. 11(11):e0166080). Western Blotting Analysis: A representative lot detected MYH-1 in Western Blotting applications (Sawano, S., et. Al. (2016). PLoS One. 11(11):e0166080). Immunohistochemistry Analysis: A representative lot detected MYH-1 in Immunohistochemistry applications (Sawano, S., et. Al. (2016). PLoS One. 11(11):e0166080). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 29 amino acids from the C-terminal half of murine Myosin-1. |
| Clone | 6F12H3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 6F12H3 is a rat monoclonal antibody that detects Myosin-1 in mouse and rat muscles. It target an epitope within the coiled coil domain in the C-terminal half. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Mouse, Rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~220 kDa observed; 223.34 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting in mouse soleus muscle tissue lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected MYH-1 in mouse soleus muscle tissue lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |