Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IF, IH(P), WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT849-100UG |
| Description | Anti-Myosin 7 (MYH7) Antibody, clone 4B51E8 |
| Alternate Names |
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| Background Information | Myosin-7 (UniProt: Q91Z83; also known as Myosin heavy chain 7, Myosin heavy chain slow isoform, MyHC-slow, Myosin heavy chain, cardiac muscle beta isoform, MyHC-beta) is encoded by the MYH7 gene (Gene ID: 140781) in murine species. Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Myosins form regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle. Myosin-7 is a component of thick filaments of the myofibrils. It contains actin- and ATP-binding sites in its conserved catalytic head domain. Its actin binding region is localized to amino acids 655-677 and 757-771 and the ATP-binding region is localized to amino acids 178-185. Myosin-7 has an N-terminal SH3-like domain (aa 32-81), a myosin motor domain (aa 85-778), and an IQ domain (aa 781-810). Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces one light meromyosin (LMM) and one heavy meromyosin (HMM). HMM can be further cleaved into two globular sub fragments (S1) and 1 rod-shaped sub fragment (S2). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Myosin 7 (MYH7), clone 4B51E8, Cat. No. MABT849, is a rat monoclonal antibody that detects Mysoin-7 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution from a representative lot detected Myosin 7 (MYH7) in mouse skeletal muscle tissue. Immunohistochemistry Analysis: A representative lot detected Myosin 7 (MYH7) in Immunohistochemistry applications (Sawano, S., et. Al. (2016). PLoS One. 11(11):e0166080). Immunofluorescence Analysis: A representative lot detected Myosin 7 (MYH7) in Immunofluorescence applications (Sawano, S., et. Al. (2016). PLoS One. 11(11):e0166080). Western Blotting Analysis: A representative lot detected Myosin 7 (MYH7) in Western Blotting applications (Sawano, S., et. Al. (2016). PLoS One. 11(11):e0166080). Western Blotting Analysis: 2 µg/mL from a representative lot detected Myosin 7 (MYH7) in mouse soleus muscle tissue lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 39 amino acids from the C-terminal region of murine Mysoin-7. |
| Clone | 4B51E8 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 4B51E8 is a rat monoclonal antibody that detects murine Myosin-7. It targets an epitope within 39 amino acids from the C-terminall half. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. Predicted to react with Human, Rat based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | 222.88 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in mouse heart tissue. Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution of this antibody detected Myosin 7 (MYH7) in mouse heart tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from the date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |