Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, IF, IHC, FACS | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT857-25UG |
| Description | Anti-Muscle Satellite Cells Antibody, clone SM C-2.6 |
| Background Information | Skeletal muscle satellite cells are quiescent mononucleated myogenic cells that reside between the sarcolemma and basement membrane of terminally-differentiated muscle fibers. They serve as a reserve population of cells with stem-like properties. The number of satellite cells are shown to be highest in postnatal muscle, but declines with age. In their quiescent stage, satellite cells are rounded and mononucleated, and do not express relevant amounts of myogenic proteins. Also, in their quiescent state they are shown to be c-kit-, Sca-1-, CD34+, and CD45-. Satellite cells can proliferate in response to injury and regenerate muscle and produce more satellite cells. Hence, they have been considered to be the only myogenic source for the maintenance of skeletal muscle. When muscle regeneration is initiated, satellite cells changes from their quiescent state to an active stage and begin to proliferative. They can proliferate and then either fuse with each other to form myotubes, which mature into myofibers, or they can fuse with damaged segments of muscle fibers. (Ref.: Fukuda, S et Al. (2004). Exp. Cell Res. 296(2); 245-255; Morgan JE and Partridge, TA (2003). Int. J. Biochem. Cell Biol. 35(8); 1151 1156). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Muscle Satellite Cells, clone SM C/2.6, Cat. No. MABT857, is a mouse monoclonal antibody that detects muscle satellite cells and has been tested for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Fluorescence Activated Cell Sorting (FACS). |
| Key Applications |
|
| Application Notes | Fluorescence Activated Cell Sorting (FACS) Analysis: A representative lot detected Muscle Satellite Cells in Fluorescence Activated Cell Sorting (FACS) applications (Urbani, L., et. Al. (2012). PLoS One. 7(11):e49860). Flow Cytometry Analysis: A representative lot detected Muscle Satellite Cells in Flow Cytometry applications (Ikemoto, M., et. Al. (2007). Mol Ther. 15(12):2178-85; Fukada, S., et. Al. (2004). Exp Cell Res. 296(2):245-55). Immunohistochemistry Analysis: A representative lot detected Muscle Satellite Cells in Immunohistochemistry applications (Fukada, S., et. Al. (2004). Exp Cell Res. 296(2):245-55; Yao, Y., et. Al. (2016). Nat Commun. 7:11415). Immunofluorescence Analysis: A representative lot detected Muscle Satellite Cells in Immunofluorescence applications (Fukada, S., et. Al. (2004). Exp Cell Res. 296(2):245-55). |
| Biological Information | |
|---|---|
| Immunogen | C2/4 myogenic cells. |
| Clone | SM-C-2.6 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone SM/C-2.6 detects quiescent satellite cells from adult mouse muscle. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein g purified |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in C2C12 cells. Flow Cytometry Analysis: 1 µg of this antibody detected Muscle Satellite Cells in a population of one million C2C12 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |