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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT882 |
| Description | Anti-Prodynorphin (Dynorphin B29) Antibody, clone Dyn9 |
| Alternate Names |
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| Background Information | Proenkephalin-B (UniProt P06300; also known as Beta-neoendorphin-dynorphin, Preprodynorphin) is encoded by the Pdyn gene (Gene ID 29190) in rat species. Proenkephalin B is an opioid polypeptide hormone involved with chemical signal transduction and cell communication. Prodynorphin is a basic building-block of endorphins, the chemical messengers in the brain that appear most heavily involved in the anticipation and experience of pain and the formation of deep emotional bonds critical in learning and memory. Prodynorphin is produced with a signal peptide (a.a. 1-21) and a propeptide (a.a. 22-163) sequence. Following the removal of the signal and propeptide sequences, the protein is further processed by proeolytic cleavages to form Alpha-neoendorphin (a.a. 166-175), Beta-neoendorphin (a.a. 166-174), Big dynorphin (a.a. 202-233; Big Dyn), Dynorphin A(1-17) (a.a. 202-218; Dyn-A17; Dynorphin A); Dynorphin A(1-13) (a.a. 202-214), Dynorphin A(1-8) (a.a. 202-209), Leu-enkephalin (a.a. 202-206 & 221-225), Leumorphin (Dynorphin B-29) (a.a. 221-248), and Rimorphin (a.a. 221-233; Dynorphin B; Dyn-B; Dynorphin B(1-13)). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-Prodynorphin (Dynorphin B29), clone Dyn9 Antibody, Cat. No. MABT882 is validated for use in Immunohistochemistry (Paraffin) for the detection of prodynorphin and leumorphin/dynorphin B-29. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:20 dilution from a representative lot detected Prodynorphin/Dynorphin B29 immunoreactivity in human midbrain tissue sections. Immunohistochemistry Analysis: A 1:20 dilution of clone Dyn9 hybridoma culture supernatant detected Prodynorphin/Dynorphin B29 immunoreactivity in human (hippocampus and cerebral cortex), mouse (fundus striati), and rat (nucleus supraopticus, lateral hipothalamic nucleus, paraventricular nucleus, and nucleus accumbens, septum pellucidum, caudate-putamen, and hippocampus) brain tissue sections (Courtesy of Ricardo Martínez Murillo, PhD, Cajal Institute, Madrid Spain). |
| Biological Information | |
|---|---|
| Immunogen | Thyroglobulin-conjugatged linear peptide corresponding to rat leumorphin (dynorphin B-29) C-terminal half sequence. |
| Epitope | C-terminus |
| Clone | Dyn9 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone Dyn9 recognizes preproenkephalin-B (a.a. 1-248 of rat sequence), proenkephalin-B (a.a. 22-248 of rat sequence), and leumorphin/dynorphin B-29 (a.a. 221-248 of rat sequence), but not other peptides derived from Proenkephalin-B. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | 28.08/28.06/28.39 kDa (Rat/Mouse/Human preproenkephalin-B), 25.85/25.76/26.28 kDa (Rat/Mouse/Human proenkephalin-B), 3.320/3.320/3.352 kDa (Rat/Mouse/Human leumorphin) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human thalamus tissue sections. Immunohistochemistry Analysis: A 1:20 dilution of this antibody detected Prodynorphin/Dynorphin B29 immunoreactivity in human thalamus tissue sections. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |