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Millipore Anti-Cytokeratin 19, Clone Troma-3 Antibody, Cat. No. Mabt913-25ug, Validated For Use In Western Blotting And Electron Microscopy, Immunohistochemistry (Paraffin), Immunoprecipitation - Mill (Additional S&H or Hazmat Fees May Apply)
Keratin, type I cytoskeletal 19 (UniProt: P19001; also known as Cytokeratin-19, CK-19, Keratin-19, K19) is encoded by the Krt19 (also known as Krt1-19) gene (Gene ID: 16669) in murine species. CK-19 belongs to the family of intermediate filament (IF) family, but differs from other IF proteins in lacking the C-terminal tail domain. About 20 different cytokeratin proteins have been reported in higher animals in varying combinations during various stages of development. CK-19 is normally expressed in the lining of the gastroenteropancreatic and hepatobiliary tracts. It is expressed throughout embryonic development with highest levels observed at 8.5 days post coitum. Its expression is shown to decrease by day 11.5 and then is increased again by 17.5 days post coitum. CK-19 is shown to be involved in the organization of myofibrils and together with keratin 8, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK-19 has been shown to be an independent prognostic factor for pancreatic neuroendocrine tumors, especially the insulin-negative tumors. TROMA 3 has been found to stain trophoblastoma TDM.1 cells, a subpopulation of the retinoic acid.-induced F9 and PCC3/ND1 cells, and in 5-azacytidine-treated 1246 cells.
Product Information
Format
Purified
Presentation
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Cytokeratin 19, clone TROMA-3 Antibody, Cat. No. MABT913-25UG, validated for use in Western Blotting and Electron Microscopy, Immunohistochemistry (Paraffin), Immunoprecipitation and Immunofluorescence.
Key Applications
Western Blotting
Electron Microscopy
Immunohistochemistry (Paraffin)
Immunoprecipitation
Immunofluorescence
Application Notes
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Cytokeratin 19 in mouse lung tissue.
Biological Information
Immunogen
Purified intermediate filaments from DM1 (Trophoblatoma) cell line.
Concentration
Please refer to lot specific datasheet.
Host
Rat
Specificity
Clone TROMA-3 detects cytokeratin 19 in human and murine cells.
Isotype
IgG2a
Species Reactivity
Human
Mouse
Species Reactivity Note
Mouse, Human.
Antibody Type
Monoclonal Antibody
Gene Symbol
Krt19
Krt1-19
Purification Method
Protein G purified
UniProt Number
P19001
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in MCF-7 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
