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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAC132 |
| Replaces | 411423-400UG |
| Description | Mouse Anti-Human IgA, Secretory Antibody, Clone 4G10.1 |
| Background Information | The Mouse Anti-Human IgA antibody is an IgG monomeric immunoglobulin, built of two heavy chains and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). It is affinity-purified and demonstrated to react with Human IgA with minimal or no cross-reaction to Human IgG or Human IgM. The target of this antibody, Human IgA, constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B-cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Mouse Anti-Human IgA, Secretory Antibody, Clone 4G10.1 is validated for use in Enzyme Immunoassay (ELISA) for the detection of Mouse Human IgA, Secretory. |
| Key Applications |
|
| Application Notes | ELISA. Can be used as a capture antibody for ELISA at 10 μg/ml in PBS without carrier protein. Antibody should be titrated for optimal results in individual systems. |
| Biological Information | |
|---|---|
| Immunogen | Purified antibody corresponding to Human IgA, Secretory. |
| Clone | 4G10.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Based on ELISA analysis, this antibody recognizes human IgA secretory subunit, with minimal or no cross-reaction human IgG or IgM. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human IgA. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein G Purified |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by ELISA against Human IgA, Human IgG and Human IgM |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 400 µg |