Miltenyi Biotec Identification and Enumeration of Themis+ Cells By Flow Cytometry; 30 Tests in 60 µL

Miltenyi Biotec Clone REA463 recognizes the human thymocyte-expressed molecule involved in selection (THEMIS) antigen, a 73 kDa signaling protein, that is also known as thymocyte selection pathway associated (TSEPA), signaling phosphoprotein specific for T cells (SPOT), or GRB2-associated protein (GASP). THEMIS is rapidly tyrosine-phosphorylated upon TCR ligation and is required for the differentiation of immature CD4/CD8 double positive thymocytes into mature CD4 or CD8 single positive thymocytes. It is expressed only in the T cell lineage and is first detected in CD4/CD8 double negative thymocytes, reaching maximum levels at the CD4/CD8 double positive stage. Following thymocyte selection, THEMIS expression is decreased in mature CD4 and CD8 single positive thymocytes and peripheral T cells. Themis−/− mice show impaired positive selection during thymocyte development and severe reduction of mature thymocytes and peripheral T cells. | Additional information: Clone REA463 displays negligible binding to Fc receptors. | | - MLTY (Additional S&H or Hazmat Fees May Apply)