Miltenyi Biotec Identification and Enumeration of Cd154+ Cells By Flow Cytometry; 30 µg in 200 µL

Miltenyi Biotec Clone REA785 specifically recognizes the mouse CD154 antigen, a 39 kDa transmembrane glycoprotein also known as CD40L, gp39, T-BAM, TRAP, or Ly-62. CD154 is transiently up-regulated on activated CD4+ T cells and plays an important role as a costimulatory molecule in T cell/antigen–presenting cell interactions through ligation of CD40. Due to its transient expression within hours after activation, CD154 can be used as a marker for activated antigen-specific CD4+ T cells. Adding a CD40-blocking antibody during the stimulation of cell suspensions prevents the down-regulation of CD154. Blocking of CD40 is not required for intracellular detection of CD154 expression or if a pure population of enriched T cells is used. | Additional information: Clone REA785 displays negligible binding to Fc receptors. - MLTY (Additional S&H or Hazmat Fees May Apply)