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Sigma-Aldrich Anti-Cyclin E Antibody, clone HE12
List Price $216.45 Your Price $216.45
Sigma-Aldrich Anti-Cyclin E Antibody Clone He12 - SIAL (Additional S&H Or Hazmat Fees May Apply)
NETA PART: SIAL-05-363-25UG
MFG.PART: 05-363-25UG
UNSPSC: 12352203
Manufacturer: Sigma-Aldrich
biological source
mouse
Quality Level
100
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HE12, monoclonal
species reactivity
human
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
General description
Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Specificity
human p52cyclin E
Immunogen
Recombinant human cyclin E
Application
Anti-Cyclin E Antibody, clone HE12 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin E & has been validated in IP, WB, ICC.
Quality
routinely evaluated in immunoblot on human A431 lysate
Target description
52 kDa
Linkage
Replaces: 04-223
Physical form
Format: Purified
Storage and Stability
2 years at -20°C or 6 months at 4°C
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
wgk_germany
WGK 1
| SKU | SIAL-05-363-25UG |
|---|---|
| Supplier Part Number | 05-363-25UG |
| UM | PK |
| UNSPSC | 12352203 |
| Manufacturer | Sigma-Aldrich |
| Temperature | F0 |
| ProductLine | SIAL |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |