Sigma-Aldrich Anti-Histone H3 Antibody, CT, pan

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Broad species cross-reactivity expected due to sequence homology.

Recognizes C-Terminal region of Histone H3, Mr 17 kDa

KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.

Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate chromatin from yeast.

Use Anti-Histone H3 Antibody, CT, pan (rabbit polyclonal antibody) validated in ChIP, WB to detect Histone H3 also known as H3, Histone H3, H3 histone family member T, histone 3 H3, histone cluster 3 H3.

Routinely evaluated by immunoblot on acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.

Western Blot Analysis:
1:25,000-1:50,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.

Approx. 17 kDa

Rabbit antiserum diluted 1:2 in storage buffer (0.02 M Phosphate, 0.25 M NaCl, 0.1% sodium azide) before the addition of glycerol to 30%.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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