Cell Signaling Irak1 (D51g7) Rabbit mAb (Biotinylated)

Name: Cell Signaling Irak1 (D51g7) Rabbit mAb (Biotinylated)
SKU: CSIG-11123S
Price: 454.50 USD

List Price $454.50 Your Price $454.50

Cell Signaling Irak1 (D51g7) Rabbit mAb (Biotinylated) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-11123S

MFG.PART: 11123S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	78-105
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Specificity/Sensitivity	IRAK1 (D51G7) Rabbit mAb (Biotinylated) detects endogenous levels of total IRAK1 protein.
Species Reactivity	Human, Mouse, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse IRAK1 protein.
Background	Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3).

Specifications