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Cell Signaling Tnf-Alpha (D2d4) Xp Rabbit mAb (Mouse Specific)
List Price $162.61 Your Price $162.61
Cell Signaling Tnf-Alpha (D2d4) Xp Rabbit mAb (Mouse Specific) - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSIG-11948T
MFG.PART: 11948T
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 20 µl |
| Reactivity | M |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 17,25,28 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:100 - 1:400, Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | TNF-α (D2D4) XP® Rabbit mAb recognizes endogenous levels of total TNF-α protein. |
| Species Reactivity | Mouse |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a recombinant mouse TNF-α protein. |
| Background | TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).Mouse and rat TNF-α are predicted to be glycosylated, while human TNF-α is not (4). |
| SKU | CSIG-11948T |
|---|---|
| Supplier Part Number | 11948T |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |