Cell Signaling Mapkapk-2 (D1e11) Rabbit mAb

Name: Cell Signaling Mapkapk-2 (D1e11) Rabbit mAb
SKU: CSIG-12155S
Price: 420.16 USD

List Price $420.16 Your Price $420.16

Cell Signaling Mapkapk-2 (D1e11) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-12155S

MFG.PART: 12155S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H M R Mk B Pg
Sensitivity	Endogenous
Molecular Weight (kDa)	47, 49 (Phosphorylated)
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	MAPKAPK-2 (D1E11) Rabbit mAb recognizes endogenous levels of total MAPKAPK-2 protein. This antibody is not known to cross-react with other MAPKAPK proteins.
Species Reactivity	Human, Mouse, Rat, Monkey, Bovine, Pig
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro381 of human MAPKAPK-2 protein.
Background	In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).