Cell Signaling Malic Enzyme 2 Antibody

Name: Cell Signaling Malic Enzyme 2 Antibody
SKU: CSIG-12399S
Price: 320.17 USD

List Price $320.17 Your Price $320.17

Cell Signaling Malic Enzyme 2 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-12399S

MFG.PART: 12399S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	65
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Malic Enzyme 2 Antibody recognizes endogenous levels of total malic enzyme 2 protein.
Species Reactivity	Human, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly450 of human malic enzyme 2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Cytosolic malic enzyme (ME1) catalyzes the conversion of malate and NADP+ to pyruvate and NADPH (1,2). NADPH is then used for fatty acid biosynthesis and lipogenesis (1,2). Cytosolic malic enzyme was shown to mediate high fat diet-induced adiposity (1). Mitochondrial malic enzyme (ME2) preferentially uses NAD+ to catalyze the conversion of malate to pyruvate with the concomitant generation of NADH (2). Recent studies have demonstrated that the tumor suppressor p53 regulates cell metabolism and proliferation by repressing the expression of both cytosolic malic enzyme and mitochondrial malic enzyme (3).