Cell Signaling Troponin I (D6f8) Rabbit mAb

Name: Cell Signaling Troponin I (D6f8) Rabbit mAb
SKU: CSIG-13083S
Price: 342.39 USD

List Price $342.39 Your Price $342.39

Cell Signaling Troponin I (D6f8) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-13083S

MFG.PART: 13083S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	28
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Troponin I (D6F8) Rabbit mAb recognizes endogenous levels of total troponin I protein.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human troponin I protein.
Background	Troponin, working in conjunction with tropomyosin, functions as a molecular switch that regulates muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca2+ sensitivity by reducing the Ca2+ binding affinity of TnC (1-3).

Specifications