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Cell Signaling Jarid2 (D6m9x) Rabbit mAb
List Price $153.52 Your Price $153.52
Cell Signaling Jarid2 (D6m9x) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSIG-13594T
MFG.PART: 13594T
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 20 µl |
| Reactivity | H M |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 150 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:200, Chromatin IP: 1:50, Chromatin IP-seq: 1:50, CUT&RUN: 1:50} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | JARID2 (D6M9X) Rabbit mAb recognizes endogenous levels of total JARID2 protein. |
| Species Reactivity | Human, Mouse |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp1114 of human JARID2 protein. |
| Background | Jumonji/ARID domain-containing protein 2 (JARID2) is a founding member of the JmjC-domain-containing protein family that is involved in regulation of histone methyltransferase activity (1,2). While many proteins in this family are protein demethylases, JARID2 lacks several conserved residues in the catalytic domain and does not exhibit protein demethylase activity (1,2). Research studies indicate that JARID2 is a nuclear protein that is highly expressed in poorly differentiated and actively dividing cells, with expression decreasing upon cellular differentiation (3,4). Expression of JARID2 protein is essential for embryonic development as the protein plays an important role in regulation of heart and liver development, neural tube fusion, and hematopoiesis (4). JARID2 is an accessory component of the polycomb repressor complex 2 (PRC2), which represses target gene expression through methylation of histone H3 at lysine 27 by EZH2 methyltransferase (5-10). JARID2 recruits the PRC2 complex to target genes and increases EZH2 methyltransferase activity by binding to nucleosomes and DNA (5-10). Additional studies show that loss of JARID2 expression results in decreased recruitment of PRC2, decreased methylation of histone H3 at lysine 27 at target genes, and delayed and incomplete differentiation of embryonic stem cells (5-10). Experimental knockdown of JARID2 in Xenopus laevis impairs the induction of gastrulation genes in blastula embryos and results in differentiation failure (5). |
| SKU | CSIG-13594T |
|---|---|
| Supplier Part Number | 13594T |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |