Cell Signaling Sv40 Large T Antigen (D1e9e) Rabbit mAb

Name: Cell Signaling Sv40 Large T Antigen (D1e9e) Rabbit mAb
SKU: CSIG-15729S
Price: 342.39 USD

List Price $342.39 Your Price $342.39

Cell Signaling Sv40 Large T Antigen (D1e9e) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-15729S

MFG.PART: 15729S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	Vir
Sensitivity	Endogenous
Molecular Weight (kDa)	90
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:100 - 1:400}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	SV40 Large T Antigen (D1E9E) Rabbit mAb recognizes transgenic levels of total SV40 Large T Antigen protein.
Species Reactivity	Virus
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys315 of SV40 Large T Antigen protein.
Background	Simian Virus 40 (SV40) is a small double-stranded, circular DNA tumor virus encoding three tumor antigens: large T, Small T, and 17KT. These antigens contribute to cellular transformation, an area of research where they are utilized extensively. Transformation of cells by SV40 large T antigen is mediated by its binding to the tumor suppressor proteins RB and p53 in the nucleus. Additional binding partners include p300, CBP, Cul7, IRS1, Bub1, Nbs1, and Fbw7, which also contribute to transformation (1).

Specifications