Cell Signaling Eif4e (C46h6) Rabbit mAb (Biotinylated)

Name: Cell Signaling Eif4e (C46h6) Rabbit mAb (Biotinylated)
SKU: CSIG-16824S
Price: 469.65 USD

List Price $469.65 Your Price $469.65

Cell Signaling Eif4e (C46h6) Rabbit mAb (Biotinylated) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-16824S

MFG.PART: 16824S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	25
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 136 mm NaCl, 2.6 mm KCI, 12 mm sodium phosphate (pH 7.4) dibasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	eIF4E (C46H6) Rabbit mAb (Biotinylated) detects endogenous levels of total eIF4E protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human eIF4E protein.
Background	Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.