Cell Signaling Otud5 (D8y2u) Rabbit mAb

Name: Cell Signaling Otud5 (D8y2u) Rabbit mAb
SKU: CSIG-20087S
Price: 342.39 USD

List Price $342.39 Your Price $342.39

Cell Signaling Otud5 (D8y2u) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-20087S

MFG.PART: 20087S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	75
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	OTUD5 (D8Y2U) Rabbit mAb recognizes endogenous levels of total OTUD5 protein.
Species Reactivity	Human, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human OTUD5 protein.
Background	Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes and deubiquitinating enzymes, respectively (1,2). Deubiquitinating enzymes (DUBs) are categorized into five subfamilies based on catalytic domain structure: USP, OTU, MJD, UCH, and JAMM/MPN. OTUD5, also known as DUBA, belongs to the OTU family of DUBs. Phosphorylation of OTUD5 at Ser177 is required to activate its DUB activity (3). Research studies have shown that OTUD5 plays a pivotal role in negatively regulating innate immune responses though its ability to bind and deubiquitinate K63-linked TRAF3, thereby suppressing production of IFN-I by macrophages (4). There is also evidence that OTUD5 participates in the adaptive immune response by negatively regulating the production of pro-inflammatory IL-17 by Th17 cells (5).

Specifications