Cell Signaling Eif4a (C32b4) Rabbit mAb

Name: Cell Signaling Eif4a (C32b4) Rabbit mAb
SKU: CSIG-2013T
Price: 148.47 USD

List Price $148.47 Your Price $148.47

Cell Signaling Eif4a (C32b4) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2013T

MFG.PART: 2013T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	48
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	eIF4A (C32B4) Rabbit mAb detects endogenous levels of total eIF4A protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met316 of human eIF4A protein.
Background	A variety of factors contribute to the important biological event of initiation of translation. The eIF4F complex of translation initiation factors binds to the 5 m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5 mRNA untranslated region to mediate ribosome binding (2,3). In addition, eIF4A has recently been shown to repress Dpp/BMP signalling in a translation-independent manner in Drosophila (4,5).